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#include <cstdio>
#include <cstdlib>
#define BAD_ARGS 1
#define FILE_ERROR 2
#define BAD_FORMAT 3
/**/
#define DEBUG
//*/
/**/
#define HOMOLOGY_TEST
//*/
/*/
#define TEST_EQUIV
//*/
struct element
{
int start1,start2;
struct element * next;
};
typedef struct element node;
int seqlength(FILE *file,int *size);
int readseq(FILE *file,char *seq,int size);
int findSeq(FILE *file,const char *input);
int equiv(char c1,char c2);
int backcheck(node **head, node **curr,int *num1, int *num2, int cursize,int maxsize);
int main (int argc, const char* argv[])
{
int count,minsize,maxsize,num1,num2,size1,size2,cursize,selector;
char flags,*ptr1,*ptr2,*ptr3, *seq1, *seq2, hashline[82]={"################################################################################\n"}, equalline[82]={"================================================================================\n"};
const char *input1, *input2, *output;
FILE *file;
node **head,**curr,*newnode;
count=1;
flags=0;
input1=NULL;
input2=NULL;
output=NULL;
seq1=NULL;
seq2=NULL;
ptr1=NULL;
ptr2=NULL;
ptr3=NULL;
minsize=0;
maxsize=0;
size1=0;
size2=0;
num1=0;
num2=0;
//==============================input handling==============================
#define INPUT_HANDLING
if(!(argc%2))
{
puts("Number of arguments is wrong, indicating an input error.");
puts(
"Incorrect arguments!\n"
"correct argument formats are:\n"
"-i filename1\n-j filename2\n"
"-o outputfilename\n"
"-m min size of match\n"
"-M max size of match\n"
"repeats are not permitted"
);
return BAD_ARGS;
}
while(count<argc&&!(flags&0x01))
{
switch(argv[count][0])
{
case '-':
switch(argv[count][1])
{
case 'i':
if(argv[count][2]||flags&0x02)
{
flags=flags|0x01;
break;
}
count++;
input1=argv[count];
count++;
flags=flags|0x02;
break;
case 'j':
if(argv[count][2]||flags&0x04)
{
flags=flags|0x01;
break;
}
count++;
input2=argv[count];
count++;
flags=flags|0x04;
break;
case 'o':
if(argv[count][2]||flags&0x08)
{
flags=flags|0x01;
break;
}
count++;
output=argv[count];
count++;
flags=flags|0x08;
break;
case 'm':
if(argv[count][2]||flags&0x10)
{
flags=flags|0x01;
break;
}
count++;
sscanf(argv[count],"%d",&minsize);
count++;
flags=flags|0x10;
break;
case 'M':
if(argv[count][2]||flags&0x20)
{
flags=flags|0x01;
break;
}
count++;
sscanf(argv[count],"%d",&maxsize);
count++;
flags=flags|0x20;
break;
default:
flags=flags|0x01;
break;
}
break;
default:
flags=flags|0x01;
break;
}
}
if(flags!=0x3E)
{
if(!(flags&0x02)){puts("missing -i argument");}
if(!(flags&0x04)){puts("missing -j argument");}
if(!(flags&0x08)){puts("missing -o argument");}
if(!(flags&0x10)){puts("missing -m argument");}
if(!(flags&0x20)){puts("missing -M argument");}
puts(
"Incorrect arguments!\n"
"correct argument formats are:\n"
"-i filename1\n-j filename2\n"
"-o outputfilename\n"
"-m min size of match\n"
"-M max size of match\n"
"repeats are not permitted"
);
return BAD_ARGS;
}
if(!(minsize>0)||!(maxsize>0))
{
puts("Min and max sizes must be positive numbers.");
return BAD_ARGS;
}
if(minsize>maxsize)
{
puts("Min size must be less than or equal to max size.");
return BAD_ARGS;
}
#ifdef DEBUG
printf("input1: %s\n""input2: %s\n""output: %s\n""minsize: %d\n""maxsize: %d\n",input1,input2,output, minsize, maxsize);
#endif
//==============================File Reading==============================
//==========File1==========
#define FILE_1
file=fopen(input1,"r");
if(!file)
{
printf("Error opening %s!\n""Terminating program\n",input1);
return FILE_ERROR;
}
flags=findSeq(file,input1);
if(flags)
{
return flags;
}
flags=seqlength(file,&size1);
if(flags)
{
return flags;
}
//==allocate space for sequence, check validity of format, and store==
seq1=(char*)malloc(sizeof(char)*(size1+1));
flags=readseq(file,seq1,size1);
if(flags)
{
return flags;
}
fclose(file);
file=NULL;
#ifdef DEBUG
printf("%s\n",seq1);
#endif
//==========File2==========
#define FILE_2
file=fopen(input2,"r");
if(!file)
{
printf("Error opening %s!\n""Terminating program\n",input2);
return FILE_ERROR;
}
flags=findSeq(file,input2);
if(flags)
{
return flags;
}
flags=seqlength(file,&size2);
if(flags)
{
return flags;
}
//==allocate space for sequence, check validity of format, and store==
seq2=(char*)malloc(sizeof(char)*(size2+1));
flags=readseq(file,seq2,size2);
if(flags)
{
return flags;
}
fclose(file);
file=NULL;
#ifdef DEBUG
printf("%s\n",seq2);
#endif
#ifdef TEST_EQUIV
//=======Begin Test=======
//This code block tests the equiv function that determines base-pair homology.
ptr1=(char*)malloc(sizeof(char)*17);
ptr1[0]='A';
ptr1[1]='C';
ptr1[2]='G';
ptr1[3]='T';
ptr1[4]='U';
ptr1[5]='R';
ptr1[6]='Y';
ptr1[7]='K';
ptr1[8]='M';
ptr1[9]='S';
ptr1[10]='W';
ptr1[11]='B';
ptr1[12]='D';
ptr1[13]='H';
ptr1[14]='V';
ptr1[15]='N';
ptr1[16]=0;
//"ACGTURYKMSWBDHVN"
ptr2=ptr1;
while(*ptr2)
{
ptr3=ptr1;
while(*ptr3)
{
printf("equiv(%c,%c)=%d\n",*ptr2,*ptr3,equiv(*ptr2,*ptr3));
ptr3++;
}
ptr2++;
}
free(ptr1);
ptr1=NULL;
ptr2=NULL;
ptr3=NULL;
//=======End Test=======
#endif
//==============================Sequence Analysis==============================
#define ANALYSIS
cursize=maxsize;
//==================Initialize head and set pointers to NULL===================
head=(node**)malloc(sizeof(node*)*(maxsize-minsize+1));
curr=(node**)malloc(sizeof(node*)*(maxsize-minsize+1));
num1=0;
while(num1<=(maxsize-minsize+1))
{
head[num1]=NULL;
curr[num1]=NULL;
num1++;
}
while(!(cursize<minsize))
{
num1=0;
while(num1<(size1-cursize))
{
num2=0;
while(num2<(size2-cursize))
{
/*
*struct element
*{
*int start1,start2;
*struct element * next;
*};
*typedef struct element node;
*/
count=0;
flags=1;
//==========Check if regions are homologous==========
while((count<cursize)&&(flags))
{
flags=equiv(seq1[num1+count],seq2[num2+count]);
count++;
}
//==========Check if regions are encompassed by Larger regions already found==========
if(flags&&(cursize<maxsize))
{
flags=backcheck(head,curr,&num1,&num2,cursize,maxsize);
}
//==========Store info about region in a node==========
if(flags)
{
newnode=(node*)malloc(sizeof(node));
newnode->next=NULL;
newnode->start1=num1;
newnode->start2=num2;
if(head[maxsize-cursize]==NULL)
{
head[maxsize-cursize]=newnode;
curr[maxsize-cursize]=newnode;
}
else
{
curr[maxsize-cursize]->next=newnode;
curr[maxsize-cursize]=newnode;
}
//num1=num1+count;
num2=num2+count;
}
num2++;
}
num1++;
}
#ifdef HOMOLOGY_TEST
printf("cursize: %d,num1: %d,num2: %d\n",cursize,num1,num2);
#endif
curr[maxsize-cursize]=head[maxsize-cursize];
cursize--;
}
count=0;
while(count<(maxsize-minsize+1))
{
while(curr[count])
{
#ifdef HOMOLOGY_TEST
printf("Homology found, size: %d, start1: %d,start2: %d\n",maxsize-count,curr[count]->start1+1,curr[count]->start2+1);
#endif
curr[count]=curr[count]->next;
}
curr[count]=head[count];
#ifdef HOMOLOGY_TEST
getchar();
#endif
count++;
}
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