Help with functions

I need help figuring out why it won't output my last function.

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  #include <iostream>
#include <fstream>
#include <iomanip> 
#include <string>
using namespace std;

int A=0,T=0,G=0,C=0;
const double tot = A+T+C+G;
bool countNucleotides(string filename);
void formatOutput(ostream& out, string nucleotideName, int nucleotideCount, double nucleotidePercentage);
void report();

int main()
{ 

    bool success = countNucleotides("dnaSequence.txt");
    
        if (!success){
            cout << "Error opening file.";
            return 1;
        }
	
	//constant for the total
	   const double tot = A+T+C+G;
    //output with totals and percentages - spaced evenly - correct format
    
}

bool countNucleotides(string filename)
{
    ifstream dnaSeq(filename);//open txt file
    
        if (!dnaSeq){
            
            return false;
        }
	
	    for (char ch; dnaSeq.get(ch); ) {
	        //char variable + get chars
	        
		    if (ch == 'A') //if A
			++A; //increment
		    else if (ch == 'T') //if T
			++T; //increment
		    else if (ch == 'C') //if C
			++C; //increment
		    else if (ch == 'G') //if G 
			++G; //increment
		    
		  while (ch == ' ') //non Nucleotides
		    cout << "Invalid Nucleotide"; //if not listed above
		    
	}
	//close file
	    dnaSeq.close();
    return true;
}

void formatOutput(ostream& out, string nucleotideName, int nucleotideCount, double nucleotidePercentage) {
    
    if (nucleotidePercentage < 0.0)
        cout << nucleotideName << nucleotideCount;
        
    else
        out << nucleotideName << nucleotideCount << setprecision(4) << setw(10) << nucleotidePercentage << "%";
    
}

void report(){
    
    bool countNucleotides(string filename);
    
    formatOutput(cout, "DNA sequence analysis: ", A+T+G+C, -1);
	
	formatOutput(cout, "\nAdenine: ", A, A/tot*100);

	formatOutput(cout, "\nThymine: ", T, T/tot*100);
 
	formatOutput(cout, "\nCytosine: ", C, C/tot*100);
	
	formatOutput(cout, "\nGuanine: ", G, G/tot*100);
	
    
}
Where do you call report()?
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